Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members

Gonzalo Ortiz-Álvarez (1) , Marie Daclin (1) , Asm Shihavuddin (1) , Pauline Lansade (1) , Aurélien Fortoul (1) , Marion Faucourt (1) , Solène Clavreul (2) , Maria-Eleni Lalioti (3) , Stavros Taraviras (3) , Simon Hippenmeyer (4) , Jean Livet (2) , Alice Meunier (1) , Auguste Genovesio (1) , Nathalie Spassky (1)

1 IBENS - Institut de biologie de l'ENS Paris
2 Institut de la Vision
3 Department of Physiology, Medical School [Patras, Greece]
4 IST Austria - Institute of Science and Technology [Klosterneuburg, Austria]

Adult neural stem cells and multiciliated ependymal cells are glial cells essential for neurological functions. Together, they make up the adult neurogenic niche. Using both high-throughput clonal analysis and single-cell resolution of progenitor division patterns and fate, we show that these two components of the neurogenic niche are lineally related: adult neural stem cells are sister cells to ependymal cells, whereas most ependymal cells arise from the terminal symmetric divisions of the lineage. Unexpectedly, we found that the antagonist regulators of DNA replication, GemC1 and Geminin, can tune the proportion of neural stem cells and ependymal cells. Our findings reveal the controlled dynamic of the neurogenic niche ontogeny and identify the Geminin family members as key regulators of the initial pool of adult neural stem cells.

Mots clés: GemC1; cell division; cell lineage; geminin family; multiciliated ependymal cells; multiciliogenesis; neural stem cells; radial glial cells; symmetric division

Domaines: Informatique [cs]; Traitement du signal et de l'image [eess.SP]

Gonzalo Ortiz-Álvarez, Marie Daclin, Asm Shihavuddin, Pauline Lansade, Aurélien Fortoul, et al.. Adult Neural Stem Cells and Multiciliated Ependymal Cells Share a Common Lineage Regulated by the Geminin Family Members. Neuron, 2019, 102 (1), pp.159-172.e7. ⟨10.1016/j.neuron.2019.01.051⟩. ⟨hal-02425373⟩