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Monitored eCLIP: high accuracy mapping of RNA-protein interactions
Abstract : CLIP-seq methods provide transcriptome-wide snapshots of RNA-protein interactions in live cells. Reverse transcriptases stopping at cross-linked nucleotides sign for RNA-protein binding sites. Reading through cross-linked positions results in false binding site assignments. In the 'monitored enhanced CLIP' (meCLIP) method, a barcoded biotiny-lated linker is ligated at the 5 end of cross-linked RNA fragments to purify RNA prior to the reverse transcription. cDNAs keeping the barcode sequence correspond to reverse transcription read-throughs. Read through occurs in unpredictable proportions, representing up to one fourth of total reads. Filtering out those reads strongly improves reliability and precision in protein binding site assignment.
Littérature citée [37 références]
https://hal-ens.archives-ouvertes.fr/hal-02387433
Contributeur : Mathieu Bahin Connectez-vous pour contacter le contributeur
Soumis le : vendredi 29 novembre 2019 - 17:35:25
Dernière modification le : lundi 10 janvier 2022 - 16:38:03
Contributeur : Mathieu Bahin Connectez-vous pour contacter le contributeur
Soumis le : vendredi 29 novembre 2019 - 17:35:25
Dernière modification le : lundi 10 janvier 2022 - 16:38:03
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