Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Monitored eCLIP: high accuracy mapping of RNA-protein interactions

Abstract : CLIP-seq methods provide transcriptome-wide snapshots of RNA-protein interactions in live cells. Reverse transcriptases stopping at cross-linked nucleotides sign for RNA-protein binding sites. Reading through cross-linked positions results in false binding site assignments. In the 'monitored enhanced CLIP' (meCLIP) method, a barcoded biotiny-lated linker is ligated at the 5 end of cross-linked RNA fragments to purify RNA prior to the reverse transcription. cDNAs keeping the barcode sequence correspond to reverse transcription read-throughs. Read through occurs in unpredictable proportions, representing up to one fourth of total reads. Filtering out those reads strongly improves reliability and precision in protein binding site assignment.
Liste complète des métadonnées

Littérature citée [37 références]  Voir  Masquer  Télécharger

https://hal-ens.archives-ouvertes.fr/hal-02387433
Contributeur : Mathieu Bahin <>
Soumis le : vendredi 29 novembre 2019 - 17:35:25
Dernière modification le : mardi 31 décembre 2019 - 01:01:52

Fichier

gky858.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Rémi Hocq, Janio Paternina, Quentin Alasseur, Auguste Genovesio, Hervé Le Hir. Monitored eCLIP: high accuracy mapping of RNA-protein interactions. Nucleic Acids Research, Oxford University Press, 2018, 46 (21), pp.11553-11565. ⟨10.1093/nar/gky858⟩. ⟨hal-02387433⟩

Partager

Métriques

Consultations de la notice

93

Téléchargements de fichiers

83