Accéder directement au contenu Accéder directement à la navigation
Nouvelle interface
Article dans une revue

Coordination of transcriptional and translational regulations in human cells infected by Listeria monocytogenes

Abstract : The invasion of mammalian cells by intracellular bacterial pathogens reshuffles their gene expression and functions; however, we lack dynamic insight into the distinct control levels that shape the host response. Here, we have addressed the respective contribution of transcriptional and translational regulations during a time-course of infection of human intestinal epithelial cells by an epidemic strain of Listeria monocytogenes, using transcriptome analysis paralleled with ribosome profiling. Upregulations were dominated by early transcriptional activation of pro-inflammatory genes, whereas translation inhibition appeared as the major driver of downregulations. Instead of a widespread but transient shutoff, translation inhibition affected specifically and durably transcripts encoding components of the translation machinery harbouring a 5’-terminal oligopyrimidine motif. Pre-silencing the most repressed target gene (PABPC1) slowed down the intracellular multiplication of Listeria monocytogenes, suggesting that the infected host cell can benefit from the repression of genes involved in protein synthesis and thereby better control infection.
Liste complète des métadonnées
Contributeur : Alice Lebreton Connectez-vous pour contacter le contributeur
Soumis le : dimanche 3 janvier 2021 - 18:09:19
Dernière modification le : jeudi 17 mars 2022 - 10:08:42


Distributed under a Creative Commons Paternité - Pas d'utilisation commerciale - Pas de modification 4.0 International License




Vinko Besic, Fatemeh Habibolahi, Benoit Noel, Sebastian Rupp, Auguste Genovesio, et al.. Coordination of transcriptional and translational regulations in human cells infected by Listeria monocytogenes. RNA Biology, 2020, 17 (10), pp.1492-1507. ⟨10.1080/15476286.2020.1777380⟩. ⟨hal-02308583v2⟩



Consultations de la notice


Téléchargements de fichiers