Extensive degradation of RNA precursors by the exosome in wild-type cells.

Rajani Kanth Gudipati 1, 2 Zhenyu Xu 3 Alice Lebreton 1, 4 Bertrand Séraphin 4 Lars M Steinmetz 3 Alain Jacquier 2 Domenico Libri 1
1 CGM - Centre de génétique moléculaire
2 Génétique des Interactions macromoléculaires
3 EMBL - European Molecular Biology Laboratory [Heidelberg]
4 IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire
Abstract : The exosome is a complex involved in the maturation of rRNA and sn-snoRNA, in the degradation of short-lived noncoding RNAs, and in the quality control of RNAs produced in mutants. It contains two catalytic subunits, Rrp6p and Dis3p, whose specific functions are not fully understood. We analyzed the transcriptome of combinations of Rrp6p and Dis3p catalytic mutants by high-resolution tiling arrays. We show that Dis3p and Rrp6p have both overlapping and specific roles in degrading distinct classes of substrates. We found that transcripts derived from more than half of intron-containing genes are degraded before splicing. Surprisingly, we also show that the exosome degrades large amounts of tRNA precursors despite the absence of processing defects. These results underscore the notion that large amounts of RNAs produced in wild-type cells are discarded before entering functional pathways and suggest that kinetic competition with degradation proofreads the efficiency and accuracy of processing.
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Contributeur : Alice Lebreton <>
Soumis le : mardi 2 août 2016 - 11:08:50
Dernière modification le : vendredi 28 juin 2019 - 09:38:04

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Rajani Kanth Gudipati, Zhenyu Xu, Alice Lebreton, Bertrand Séraphin, Lars M Steinmetz, et al.. Extensive degradation of RNA precursors by the exosome in wild-type cells.. Molecular Cell, Elsevier, 2012, 48 (3), pp.409-21. ⟨10.1016/j.molcel.2012.08.018⟩. ⟨hal-01350901⟩

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