Dynamic Electrochemiluminescence Imaging of Single Giant Liposomes Opening at Polarized Electrodes

Fatma Ben Trad (1) , Vincent Wieczny (1) , Jérôme Delacotte (1) , Mathieu Morel (1) , Manon Guille-Collignon (1) , Stéphane Arbault (2) , Frédéric Lemaître (1) , Neso Sojic (3) , Eric Labbé (1) , Olivier O. Buriez (1) , Ben Fatma (4, 1)

1 PASTEUR - Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640)
2 CBMN - Chimie et Biologie des Membranes et des Nanoobjets
3 ISM - Institut des Sciences Moléculaires
4 ISD - Institut Supérieur de Documentation [Manouba]

In this work, the characterization of release events from liposomes has been addressed quantitatively by an electrochemiluminescence (ECL) imaging strategy. First, ECL reagents ([Ru(bpy)3] 2+ and tripropylamine) have been encapsulated in sealed giant asymmetrical liposomes (100 µm in diameter) made of DOPG/DOPC phospholipids. After sedimentation on an ITO (Indium Tin Oxide) electrode material, the opening of liposomes was then triggered by polarization of the surface. Under these conditions, amperometry, epifluorescence and ECL were combined and synchronized to monitor and image the rupture of giant liposomes through the release and subsequent ECL emission of their redox content. Amperometry allowed the quantification of the content released from single liposomes. The location and status of liposomes (closed or opened) was assessed by epifluorescence. ECL provided imaging the efflux of matter after liposome opening. This original ECL imaging approach favorably compares to strictly photoluminescent or electrochemical techniques and appears adapted to the investigation of membrane rupture / permeation events.

Domaines: Chimie

Fatma Ben Trad, Vincent Wieczny, Jérôme Delacotte, Mathieu Morel, Manon Guille-Collignon, et al.. Dynamic Electrochemiluminescence Imaging of Single Giant Liposomes Opening at Polarized Electrodes. Analytical Chemistry, 2022, 94 (3), pp.1686-1696. ⟨10.1021/acs.analchem.1c04238⟩. ⟨hal-03552115⟩