Assessment of presumed small-molecule ligands of telomeric i-DNA by biolayer interferometry (BLI)

Hugues Bonnet (1) , Maéva Morel (1) , Alexandre Devaux (1) , Joseph Boissieras (2) , Anton Granzhan (2) , Benjamin Elias (3) , Thomas Lavergne (1) , Jérôme Dejeu (1, 4) , Eric Defrancq (1)

1 DCM - I2BM - Département de Chimie Moléculaire - Ingéniérie et Intéractions BioMoléculaires
2 CMBC - Chimie et modélisation pour la biologie du cancer
3 IMCN - Institut de la matière condensée et des nanosciences / Institute of Condensed Matter and Nanosciences
4 FEMTO-ST - Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174)

Biolayer interferometry (BLI) and circular dichroism (CD) spectroscopy were used to investigate the interaction between previously reported i-motif DNA (i-DNA) ligands and folded or unfolded i-DNA in acidic (pH 5.5) and near-neutral (pH 6.5) conditions. We observed that although several ligands, in particular macrocyclic bis-acridine (BisA) and pyridostatin (PDS), showed good affinities for the telomeric i-motif forming sequence, none of the ligands displayed selective interactions with the i-DNA structure nor was able to promote its formation.

Domaines: Chimie de coordination

Hugues Bonnet, Maéva Morel, Alexandre Devaux, Joseph Boissieras, Anton Granzhan, et al.. Assessment of presumed small-molecule ligands of telomeric i-DNA by biolayer interferometry (BLI). Chemical Communications, 2022, 58 (33), pp.5116. ⟨10.1039/D2CC00836J⟩. ⟨hal-03632613v2⟩